Salmonella Typhi/Paratyphi A Antigen Detection Kit (Colloidal Gold Method)

Salmonella Typhi/Paratyphi A Antigen Detection Kit (Colloidal Gold Method)

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Product Detail

Product Description

Intended use
Babio®Salmonella typhi/paratyphi A Antigen Detection kit (Colloidal Gold Method) is a rapid chromatographic immunoassay for the qualitative detection and differentiation of specific antibodies against specific Salmonella typhi antigen in human serum or plasma. It is intended for in vitro diagnosis of typhoid fever.

Reagents And Materials Supplied


Test Principle
Babio®Salmonella typhi/paratyphi A Antigen Detection(Colloidal Gold Method) is a qualitative one-step immunochromatographic analysis method. The test uses a mixture of monoclonal antibody/colloidal gold dye conjugates and polyclonal antibodies immobilized on a solid phase. This will selectively identify Salmonella typhi-Salmonella paratyphi antigens associated with typhoid fever with a high degree of sensitivity and specificity.

Specimen Collection
1. Serum (S): Collect whole blood into a collection tube (does not contain anticoagulants such as heparin, EDTA and sodium citrate) by venipuncture, let it stand for 30 minutes for blood coagulation, and then centrifuge the blood to obtain the supernatant Serum specimen of liquid.
2.Plasma (P): Collect whole blood in a collection tube (containing anticoagulants, such as heparin, EDTA, and sodium citrate) by venipuncture, and then centrifuge the blood to obtain a plasma sample.
3. Whole blood (WB): Collect whole blood through a blood sampling device. WB can be transferred directly to the test card by pipetting.

Test Procedure
1.Before opening the bag, please leave it at room temperature. Take the test device out of the sealed bag and use it as soon as possible. The best results will be obtained if the measurement is performed within one hour.
2.Dispense 35 µL of serum/plasma or whole blood into the sample wells of the test card.
3.Dispense 1 drop of buffer directly from the buffer bottle, or use a calibrated pipette to transfer 40 µL of buffer to the sample well. 4.The result should be between 10 and 20 minutes, but not more than 30 minutes.

Interpretation of Results
NEGATIVE:
If only the quality control line C appears, and the test lines T1 and T2 are not purple/red, it indicates that no antigen is detected, and the result is negative.
POSITIVE:
Typhi antigen positive: If both the quality control line C and the test line T1 appear purple/red, it indicates that the typhi antigen is detected, and the result is positive for typhi antigen.Paratyphi A antigen positive: If both the quality control line C and the test line T2 appear purple/red, it indicates that the paratyphi A antigen is detected, and the result is positive for paratyphi A antigen. Typhi and paratyphi A antigen positive: If the quality control line C and the test lines T1 and T2 all appear purple/red, it indicates that the typhi and paratyphi A antigen are detected, and the result is positive for both typhi and paratyphi A antigen.
INVALID:
If the quality control line C is not displayed, the test result is invalid regardless of whether there is a purple/red test line, and it should be tested again.



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