Typhoid IgG/IgM Test Kit (Colloidal Gold Method)

Typhoid IgG/IgM Test Kit (Colloidal Gold Method)

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Product Description

Babio®Typhoid IgG/IgM Test Kit (Colloidal Gold Method) is a rapid chromatographic immunoassay for the qualitative detection and differentiation of specific IgM and IgG antibodies against specific Salmonella typhi antigen in human serum or plasma. It is intended for in vitro diagnosis of typhoid fever.

Test Principle
The Babio® Typhoid IgG/IgM Test Kit (Colloidal Gold Method) is a method for qualitative detection of IgG and IgM antibodies against Streptococcus typhi in human serum, plasma or whole blood. The test provides a differential detection of anti-S. typhi-IgG and anti-S. typhi-IgM antibodies and can be used to presumptive distinction between a current, latent and/or carrier S. typhi infection. Serum, plasma or whole blood samples could have been used for this test. The specific S. typhi antigen is immobilized onto cellulose nitrate membrane as test lines. When the test sample is added to the sample pad, it migrates upwards. If IgG or IgM antibodies to S. typhi are present in the specimen they will bind to the colloidal gold-antigen conjugate. The complex will continue to move on the cellulose nitrate membrane and then captured at the test window zone by the immobilized specific S. typhi antigen, and form pale to dark lines. The intensity of the lines will vary depending on the amount of antibody present in the sample. The appearance of a colored line in a specific test region should be considered as positive for that particular antibody (IgG and/or IgM). As a control, a colored line will always appear in the control line area, indicating that the appropriate specimen volume and the appropriate membrane wick have been added.
Reagents And Materials Supplied

Test Procedure
1.Before opening the bag, please leave it at room temperature. Take the test device out of the sealed bag and use it as soon as possible. The best results will be obtained if the measurement is performed within one hour.
2.Dispense 35 µL of serum/plasma or whole blood into the sample wells of the test card.
3.Dispense 1 drop of buffer directly from the buffer bottle, or use a calibrated pipette to transfer 40 µL of buffer to the sample well.
4.The result should be between 10 and 20 minutes, but not more than 30 minutes.

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