Feline/Canine Heartworm Antigen (FCHW Ag) Test Kit is used for screening and auxiliary diagnosis of canine heart filariasis.
The intermediate hosts of canine heartworm are various mosquitoes such as Anopheles sinensis, Aedes albopictus, and Culex pipiens pallens. Dogs are infected due to being bitten by mosquitoes containing infectious larvae. The adult worms of heartworms parasitize the right heart and pulmonary arteries of dogs and humans. The adults produce larvae (which are directly produced by females and become microfilaria) that circulate in the bloodstream. After mosquitoes bite dogs infected with heartworm disease, they carry the heartworm larvae. The larvae molt in the mosquito's salivary glands and become infectious young worms, which are then infected by mosquitoes biting other dogs and humans. Dogs can be repeatedly infected with this disease. Due to the parasitic nature of adult worms in the heart, they cause heart damage and gradually affect the health of dogs and humans. Therefore, at the beginning of the disease, dogs do not experience obvious discomfort. After a period of infection, they gradually develop exercise intolerance, cough, and shortness of breath. In the past, they were often misdiagnosed as bronchitis or ordinary heart disease. In the later stage, heart and lung failure, anemia, pulmonary hydrops, ascites, jaundice, liver and kidney failure and death may occur. Dogs can develop chronic endocarditis, cardiac hypertrophy, and right ventricular dilation. In severe cases, it may be caused by venous congestion causing lesions such as ascites and liver enlargement. The symptoms of the affected dog are cough, palpitations, thin and weak pulse, and presence in the heart.Noise, increased abdominal circumference, and difficulty breathing. Anemia increases in later stages, gradually leading to emaciation and weakness until death .
This reagent kit uses double antibody sandwich immunochromatography. If the sample contains a sufficient amount of corresponding antigen, the antigen will bind to the monoclonal antibody coated with colloidal gold on the gold pad, forming an antibody antigen complex. When this complex migrates upwards to the detection area (T-line) with capillary effect, it then binds with another monoclonal antibody to form an "antibody antigen antibody" complex and gradually condenses into a visible detection line (T-line). Excess colloidal gold antibodies continue to migrate to the quality control area (C-line) and are captured by the secondary antibody to form a visible C-line. The detection results are displayed through the C-line and T-line. The red strip displayed on the quality control line (C) is the standard for determining whether the chromatography process is normal, and also serves as the internal control standard for the product.
| Components | Specification | ||
| 1T/box | 20T/box | 25T/box | |
| Reagent card | 1 | 20 | 25 |
| Diluent pipe | 1 | 20 | 25 |
| Instruction | 1 | 1 | 1 |
Note: swabs are complimentary separately according to package specifications.
【Self-contained appliance】
Timepiece
【Storage and expiration date】
The kit is stored at 2-30℃. Do not freeze. Valid for 24 months; After the kit is opened, the reagent should be used as soon as possible.
【Sample requirement】
1. Sample: Canine serum.
2. Samples should be tested on the same day; Samples that cannot be tested on the same day should be stored at 2-8 ° C, and those that exceed 24 hours should be stored at -20 ° C.
【Inspection method】
1. Before use, restore the kit to room temperature (15-30℃).
2. Remove the reagent card from the aluminum foil bag and place it on a clean platform.
3. Unscrew the top tube cap on the diluent tube cap containing the sample, invert the diluent tube, squeeze the tube wall, and add 3-5 drops of specimen mixture into the sample hole (S hole) of the reagent card.
4. The results can be read in 10-15 minutes. The result is invalid after 15 minutes.
Positive: Both the quality control line (C line) and the test line (T line) appear
Negative: Only the quality control line (C line) is available
Invalid: Quality control line does not appear, take a new device to retest
1. This product is only used for qualitative testing and does not indicate the virus level in the sample.
2. Test results of this product are for reference only and should not be used as the sole basis for diagnosis and treatment, but should be made by a physician after evaluating all clinical and laboratory evidence.
3. A negative result may occur if the viral antigen present in the sample is below the detection limit of the assay, or if the antigen detected at the disease stage at which the sample was collected is not present.
4. The operation should be carried out in strict accordance with the instructions. Do not use expired or damaged products.
5. The test card should be used within 1 hour after opening; If the ambient temperature is higher than 30 ° C or more humid, it should be used immediately.
6. If the T line has just begun to show color, and then the line color gradually fades or even disappears, in this case, the sample should be diluted several times and tested until the T line color is stable.
7. This product is a disposable product. Do not reuse it.
