Canine Rotavirus Antigen (CRV Ag) Test Kit provides rapid qualitative detection of canine rotavirus antigen, which can be used for canine rotavirus antigen infection screening and auxiliary diagnosis.
This kit was performed by double-antibody sandwich immunochromatography. If the sample contains sufficient amounts of the canine rotavirus antigen, the canine rotavirus antigen binds to a monoclonal antibody coated with colloidal gold on the gold standard pad to form an antibody-antigen complex. The complex with the capillary effect upward migration to the detection line (T line), combined with another monoclonal antibody to form "antibody-antibody-antibody" complex and gradually agglutinate into a visible detection line (T line), excessive colloidal gold antibody continue to migrate to the quality control line (C line) is captured by secondary antibody and form visible C line. Test results are shown by the C and T lines. The red band shown by the quality control line (the line C) is the standard to determine whether the chromatography process is normal, and also serves as the internal control standard of the product.
| Components | Specification | ||
| 1T/box | 20T/box | 25T/box | |
| Reagent card | 1 | 20 | 25 |
| Diluent pipe | 1 | 20 | 25 |
| Instruction | 1 | 1 | 1 |
Note: swabs are complimentary separately according to package specifications.
【Self-contained appliance】
Timepiece
【Storage and expiration date】
The kit is stored at 2-30℃. Do not freeze. Valid for 24 months; After the kit is opened, the reagent should be used as soon as possible.
【Sample requirement】
1.Sample: dog feces.
2.Samples should be tested on the same day; Samples that cannot be tested on the same day should be stored at 2-8 ° C, and those that exceed 24 hours should be stored at -20 ° C.
【Specimen collection and processing】
Please collect samples according to the diagram. Too many samples will result in false positive results.
Insert the above swab with a stool sample (the size of a mung bean) into the diluent tube and mix it well with the liquid. Finally squeeze the swab so that most of the solution remains in the extraction tube, then remove the swab and tighten the cap. It works best if the sample is tested immediately after collection.
【Inspection method】
1.Before use, restore the kit to room temperature (15-30℃).
2.Remove the reagent card from the aluminum foil bag and place it on a clean platform.
3.Unscrew the top tube cap on the diluent tube cap containing the sample, invert the diluent tube, squeeze the tube wall, and add 3-5 drops of specimen mixture into the sample hole (S hole) of the reagent card.
4.The results can be read in 10-15 minutes. The result is invalid after 15 minutes.
Positive: Both the quality control line (C line) and the test line (T line) appear
Negative: Only the quality control line (C line) is available
Invalid: Quality control line does not appear, take a new device to retest
1. This product is only used for qualitative testing and does not indicate the virus level in the sample.
2. Test results of this product are for reference only and should not be used as the sole basis for diagnosis and treatment, but should be made by a physician after evaluating all clinical and laboratory evidence.
3. A negative result may occur if the viral antigen present in the sample is below the detection limit of the assay, or if the antigen detected at the disease stage at which the sample was collected is not present.
4. The operation should be carried out in strict accordance with the instructions. Do not use expired or damaged products.
5. The test card should be used within 1 hour after opening; If the ambient temperature is higher than 30 ° C or more humid, it should be used immediately.
6. If the T line has just begun to show color, and then the line color gradually fades or even disappears, in this case, the sample should be diluted several times and tested until the T line color is stable.
7. This product is a disposable product. Do not reuse it.
