The Hematoxylin Eosin Staining Solution Kit, manufactured by Baibo Biotechnology Co., Ltd., is a premier product made in China. Baibo Biotechnology, a leading company in the field, offers customization and OEM services to meet diverse research needs. Available for online wholesale, this kit exemplifies Baibo’s robust capabilities and commitment to quality. With advanced manufacturing processes and a strong R&D foundation, Baibo Biotechnology ensures reliable and high-performance staining solutions for histological applications.
【 Product name 】
Hematoxylin Eosin Staining Solution Kit
【 Packing specification 】
The packaging specifications of each single bottle (barrel) of dyeing liquid are :20m1, 100m1, 250m1, 500m1, 1L, 5L, and the packaging specifications of the whole group of dyeing liquid are :4x20ml/ box, 4x100m1/ box, 4x250m1/ box, 4x500m1/ box, 4X1L/ box, 4X5L/ box. Liquid 1 :4x250m1/ box, liquid 2 :4x250m1/ box
【 Intended use 】
Hematoxylin Eosin Staining Solution Kit is mainly used to stain cells and tissues.
【 Test principle 】
Hematoxylin-eosin staining solution (H-E strain) is mainly used to display the normal components of various tissues and the general morphological structure of lesions for comprehensive observation. Hematoxylin-eosin staining solution is the most essential staining method in biology, histology, pathology and cytology. It is widely used in pathological diagnosis, teaching and scientific research, and has important value. The nucleus of the cell is composed of acidic substances, which have a strong affinity with the basic dye (hematoxylin), while the cytoplasm, which contains alkaline substances and an acidic dye (eosin), has a strong affinity. Therefore, after the cells or tissue sections are stained by hematoxylin and eosin staining solution, the nucleus is stained by hematoxylin into a bright blue-purple color, the cytoplasm, muscle fibers, collagen fibers, etc., are red in varying degrees, and the red blood cells are orange-red.
【 Storage Conditions and expiry date 】
The kit should be stored in a relative humidity of no more than 80%, no corrosive gases and well-ventilated room temperature environment of 5~30℃, valid for 18 months.
【 Limitation of test method 】
For nuclear histomorphology observation and staining only
【 Interpretation of test results 】
The nucleus is purple, and the cytoplasm, interstitium, and various fibers are red to varying degrees.
【 Note 】
1. It is normal to produce an oxide film on the surface of hematoxylin dye solution and a little aluminum sulfate crystal precipitation at the bottom. The oxide film should be removed before use.
The diluted lithium carbonate solution is then used for bluing.
2, when the temperature is low, the hematoxylin dye is not easy to color, and the dyeing time can be appropriately extended.
3. Color separation is to use hydrochloric acid to wash away the excessive hematoxylin adsorbed by cells, so that the nucleocytoplasm is in sharp contrast; Color separation time should not be too long, otherwise nuclear light dyeing.
4, eosin dyeing through ethanol, not easy to soak for a long time, especially with low concentration of ethanol, should avoid eosin being decolorized.
5, smear and print hematoxylin, eosin staining method is fixed after washing, from the third step and paraffin section dyeing the same.
6. The preparation of a good hematoxylin and eosin stained slice is closely related to whether it is fixed in time and sufficiently.
7. Please do not use this reagent after its validity period. When storing this kit, avoid high, low temperature and bright environment, so as not to affect the quality and effect.
8. This product is only used for external inspection and is strictly prohibited for internal administration. After use, waste should be disposed according to the requirements of hospitals or environmental protection departments.
9, production batch number, expiration date for the outer packaging.
