Feline herpesvirus antigen (FHV Ag) test kitFeline Herpesvirus antigen (FHV Ag) detection kit is used for rapid qualitative detection of feline herpesvirus antigen in cat eye and nose secretions, and can be used for screening and auxiliary diagnosis of feline herpesvirus infection
【testing principle】
This kit uses double antibody sandwich immunochromatography. If the sample contains a sufficient amount of cat blisters.The rash virus antigen, the feline herpesvirus antigen will bind to the colloidal gold coated antibody on the gold label pad.To form antibody-antigen complex. This complex migrates upward to the detection line (T-line) with the capillary effect.When, it binds to another antibody to form an "antibody-antigen-antibody" complex and gradually agglutinates into a single molecule.As seen in the detection line (T-line), excess colloidal gold antibodies continue to migrate to the quality control line (C-line) by secondary antibodies.Capture and form a visible C-line. The test results are displayed on the C and T lines. The quality control line (C line) is shown.The red strip is the standard to determine whether the chromatographic process is normal, and also serves as the internal control standard of the product quasi.
【 Package Specifications and components 】
【 Storage and expiration date 】
This kit is stored at 2-30℃; Do not freeze. Valid for 24 months; test kit After opening the bag, use the reagent as soon as possible.
[Sample requirements]
1. Detection samples: cat eye and nose secretions.
2. The sample can be stored at 2-8℃, and for more than 24 hours, it should be stored at -20℃.
【 Specimen Collection and treatment 】
Eyes: Place the sterile swab in the corner of your pet's eye and gently rotate it several times to collect the eye secretions.
Nose: Insert a sterile cotton swab parallel to the nostril and leave it for a few seconds to absorb secretions.
A nasal swab is used to collect samples for best performance.
The swab with the collected sample is inserted into the diluent tube and thoroughly mixed with the liquid.
Finally squeeze the swab so that most of the solution remains in the extraction tube, then remove the swab and tighten the bottle
Build. It works best if the sample is tested immediately after collection.
[test method]
1. Before use, restore the kit to room temperature (15-30℃).
2. Remove the reagent card from the foil bag and place it on a clean platform.
3. Unscrew the top tube cap on the diluent tube cover containing the sample, invert the diluent tube,
Squeeze the wall of the tube and add 3-5 drops of the sample mixture into the sample hole (S hole) of the reagent card.
4. The results can be read in 10-15 minutes. The result is invalid after 15 minutes.
【 Result interpretation 】
Positive: Both the quality control line (C line) and the test line (T line) appear
Negative: Only the quality control line (C line) is available
Invalid: Quality control line does not appear, take a new device to retest
